Figure 2.

(A) Expression of full-length DNAJA1 (UniProt entry P31689) in MiaPaCa2 pancreatic cancer cells suppresses the activation of c-Jun in response to anisomycin (370 nM) or UV treatment (20 or 50 J/m² doses). Twenty minutes after being treated, cells were subjected to lysis, and the levels of phospho-c-Jun (S63) and total c-Jun were evaluated by Western blotting. Expression levels of exogenously expressed His-tagged DNAJA1 were evaluated by immunoblotting with the anti-His antibody, while immunoblotting with the anti-tubulin antibody was performed as a loading control. (B) Expression of full-length DNAJA1 in MiaPaCa2 pancreatic cancer cells decreases the level of survival in response to anisomycin treatment-induced stress. The level of cell survival was measured by the MTT assay 24 h after treatment (**p < 0.01; ***p < 0.001).