(A) Transparent surface and ribbon representation of DNAJA1-JD
highlighting another proposed DnaK binding site based upon the bovine
auxilin–bovine Hsp70 complex (PDB entry 2QWN) (colored red and
green; conserved HPD motif). (B) Transparent surface and ribbon representation
of DNAJA1-JD (rotated ∼90°) with the proposed DnaK binding
site based upon NMR titration data (colored blue). (C) Transparent
surface and ribbon of DNAJA1-JD (rotated ∼90°) with the
proposed inhibition site based upon the TIM14–TIM16 complex
(colored purple). (D) Sequence of DNAJA1-JD with the proposed interaction
sites indicated: DnaK binding site from titrations (blue circle),
DnaK inhibition site (purple triangles), DnaK binding site from the
cross-linked auxilin–Hsp70 complex (red stars), and the highly
conserved HPD motif (green box). The conserved HPD motif and helix
α2, which is potentially an important component of the DnaJ–DnaK
interaction site and the TIM16-like inhibitory binding site, are labeled.