Figure 6.

(A) Overlay of 2D ¹H–¹⁵N HSQC spectra of free DNAJA1-JD (black) and DNAJA1-JD with O-phospho-l-serine (red). (B) Transparent surface representation and ribbon diagram of DNAJA1-JD bound with O-phospho-l-serine, with the residues showing a chemical shift perturbation upon binding of O-phospho-l-serine colored blue and the one residue, Glu51, that shows the greatest chemical shift perturbation with every binding ligand colored red. (C) Expanded view of O-phospho-l-serine bound to a ribbon diagram of DNAJA1-JD, where residues with side chains directed toward the ligand are displayed.