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Dependence of kcat/Km for the TIM-catalyzed turnover of the free carbonyl form of [1-13C]-GA in D2O on [HPO32–] at pD 7.0, 25 °C, and an ionic strength of 0.10 (NaCl). (A) Reactions catalyzed by the Y208T, Y208S, Y208A, and S211A mutants of yTIM. (B) Reactions catalyzed by the Y208F mutant.
