. 2014 Jan 24;53(6):1051–1058. doi: 10.1021/bi4016814

Table 1. Analyses of Multimeric Species Present in Wild-Type RePC (WT) and H216N in the Presence and Absence of Acetyl CoA, Pyruvate, and MgCl₂ from Analytical Ultracentrifugation Studies.

	monomer		dimer		tetramer
RePC enzyme	molecular mass (kDa)	%	molecular mass (kDa)	%	molecular mass (kDa)	%
WT^a	163 ± 26	14	287 ± 38	14	450 ± 32	72
H216N^a	152 ± 41	12	304 ± 51	15	499 ± 82	73
WT^b	mixture of monomers and dimers			14	472 ± 52	86
H216N^b	mixture of monomers and dimers			14	562 ± 57	86

In 0.1 M Tris-HCl (pH 7.8) and 1 mM DTE.

In 0.1 M Tris-HCl (pH 7.8), 0.1 mM acetyl CoA, 10 mM pyruvate, 5 mM MgCl₂, and 1 mM DTE.

Table 1. Analyses of Multimeric Species Present in Wild-Type RePC (WT) and H216N in the Presence and Absence of Acetyl CoA, Pyruvate, and MgCl2 from Analytical Ultracentrifugation Studies.