Table 2. Kinetic Parameters for Steady-State Reactions, Including Pyruvate Carboxylation, Oxamate-Dependent Oxaloacetate Decarboxylation, Bicarbonate-Dependent MgATP Cleavage, and Carbamoyl Phosphate-Dependent MgADP Phosphorylation Catalyzed by RePC.
| wild-type RePC | H216N | |
|---|---|---|
| pyruvate carboxylation | ||
| kcat (s–1) | 11.0 ± 0.3 | 0.052 ± 0.007 |
| oxaloacetate decarboxylation | ||
| kcat (s–1) | 0.28 ± 0.01 | 0.10 ± 0.01 |
| MgATP cleavage | ||
| kcat (s–1) | 0.0617 ± 0.0002 | 0.0117 ± 0.0007 |
| Kma (μM) | 9.0 ± 0.9 | 82 ± 16 |
| kcat/Km (mM–1 s–1) | 6.86 | 0.14 |
| MgADP phosphorylation | ||
| kcat (s–1) | 1.65 ± 0.07 | 0.022 ± 0.003 |
| Kmb (μM) | 2.8 ± 0.4 | 8.1 ± 2.4 |
| kcat/Km (mM–1 s–1) | 589 | 2.7 |
a
Km for MgATP.
b
Km for carbamoyl phosphate.