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. 2014 Jan 24;53(6):1051–1058. doi: 10.1021/bi4016814

Table 3. Analysis of the Kinetics of Single-Turnover MgATP Cleavage Reactions in Wild-Type and Mutant RePC.

enzyme Kd (μM) kcat (s–1) kcat (% of WT value)
WT 10 ± 4 1.400 ± 0.004 100
H216N 12 ± 2 0.011 ± 0.001 0.8
E218Q 11 ± 3 0.032 ± 0.003 2.2
E305A 8 ± 5 0.032 ± 0.010 2.2