Table 4. Kinetic Parameters for Binding of MgFTP to RePC and H216N and Its Dissociation from the Enzymes upon Displacement by MgATP, Determined from Fits of Single Exponentials to the Stopped-Flow Fluorescence Data in Figure 5.
kbindingobs (s–1) | kdisplacementobs (s–1) | |
---|---|---|
wild-type RePC | 302 ± 75 | 241 ± 37 |
H216N | 263 ± 27 | 254 ± 73 |