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. 2014 Mar 10;136(13):5047–5056. doi: 10.1021/ja4130059

Figure 5.

Figure 5

Reduced cellular toxicity by enhanced ZFRs. (a) Cell viability of HEK293 cells transfected with increasing amounts of expression vector of standard or eZFRs. Toxicity was normalized to 293 cells transfected with the I-SceI endonuclease. Error bars indicate standard deviation (n = 3). (b) Western blot of lysate from HEK293 cells transfected with increasing amounts of expression vector of standard ZFRs or eZFRs. Samples were taken 48 h after transfection and probed with horseradish peroxidase-conjugated anti-HA and anti-β-actin (loading control) antibodies.