Table 1. Library of CPP-ELPBC Nanopeptifiers.
nanopeptifier | CPP functionalization | MW (kDa) | RH at 37 °C (nm)a | RH at 42 °C (nm)a |
---|---|---|---|---|
Arg5-ELPBC | RRRRR | 40.50 | 5.7 ± 0.3 | 20.8 ± 1.0 |
Arg8-ELPBC | RRRRRRRR | 40.97 | 5.4 ± 0.4 | 21.2 ± 0.3 |
TAT-ELPBCb | YGRKKRRQRRR | 41.26 | 5.7 ± 0.3 | 21.4 ± 0.3 |
RTAT-ELPBCc | YGRGGRRGRRR | 41.05 | 6.5 ± 0.2 | 21.8 ± 0.3 |
ELPBC | none | 39.72 | 6.2 ± 0.1 | 20.3 ± 0.3 |
Hydrodynamic radius determined by dynamic light scattering of ELPBC at 10 μM in PBS. Data represents mean of three measurements ± SEM.
TAT sequence corresponds to residues 47–57 from the transactivator of transcription of HIV.
RTAT was modified to explore the effects of arginine architecture on CPP function, where all nonarginine residues of TAT were replaced with neutral glycine residues.