. 2014 Mar 10;14(4):2058–2064. doi: 10.1021/nl5002313

Table 1. Library of CPP-ELP_BC Nanopeptifiers.

nanopeptifier	CPP functionalization	MW (kDa)	R_H at 37 °C (nm)^a	R_H at 42 °C (nm)^a
Arg₅-ELP_BC	RRRRR	40.50	5.7 ± 0.3	20.8 ± 1.0
Arg₈-ELP_BC	RRRRRRRR	40.97	5.4 ± 0.4	21.2 ± 0.3
TAT-ELP_BC^b	YGRKKRRQRRR	41.26	5.7 ± 0.3	21.4 ± 0.3
RTAT-ELP_BC^c	YGRGGRRGRRR	41.05	6.5 ± 0.2	21.8 ± 0.3
ELP_BC	none	39.72	6.2 ± 0.1	20.3 ± 0.3

Hydrodynamic radius determined by dynamic light scattering of ELP_BC at 10 μM in PBS. Data represents mean of three measurements ± SEM.

TAT sequence corresponds to residues 47–57 from the transactivator of transcription of HIV.

RTAT was modified to explore the effects of arginine architecture on CPP function, where all nonarginine residues of TAT were replaced with neutral glycine residues.

Table 1. Library of CPP-ELPBC Nanopeptifiers.

Table 1. Library of CPP-ELP_BC Nanopeptifiers.