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| PIR1-coreFED construct | Km (μM) | Vmax (μM min–1) | kcat/Km (min–1 M–1) |
|---|---|---|---|
| C152 | 73.9 ± 17.3 | 1.8 ± 0.15 | 2.4 × 104 |
| H154A | 88.8 ± 33.3 | 0.6 ± 0.12 | 6.7 × 103 |
| N157A | 98.8 ± 20.5 | 0.65 ± 0.1 | 6.5 × 103 |
| DM | 92.0 ± 21.2 | 0.30 ± 0.1 | 3.2 × 103 |
| C152S | not available | not available | not available |
