Table 3. Kinetic Parameters for Proline Activation by WT and Mutant Ec ProRSa.
| Ec ProRS | kcat (s–1) | KM (mM) | kcat/KM (mM–1 s–1) | relative kcat/KM | fold decrease |
|---|---|---|---|---|---|
| WTb | 12.6 ± 4.9 | 0.18 ± 0.03 | 71 | 1.0 | |
| E303A | 5.2 ± 0.6 | 0.216 ± 0.004 | 24 | 0.34 | 3.0 |
| E303D | 4.6 ± 0.5 | 0.199 ± 0.005 | 23 | 0.32 | 3.1 |
| E303K | 5.1 ± 1.5 | 0.30 ± 0.03 | 17 | 0.24 | 4.2 |
| K279E | 5.7 ± 0.9 | 0.22 ± 0.04 | 26 | 0.37 | 2.7 |
| K279E/E303K | 5.9 ± 0.9 | 0.19 ± 0.01 | 31 | 0.44 | 2.3 |
a
Results are the average of three trials with the standard deviation indicated.
b
Data is from ref (47).