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. 2014 Apr 14;9(4):e94510. doi: 10.1371/journal.pone.0094510

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© 2014 Nieves Torres et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Upper panel is representative sections after hematoxylin and eosin (H&E) at the venous stenosis of the LV-shRNA-ADAMTS-1 (LV) and control shRNA (C) transduced vessels at day at day 14 showing increase in lumen vessel area. A) Lower panel is representative polarized light microscopy of picrosirius red-stained venous stenosis showing decreased fibrosis (collagen fibers are bright yellow) of the LV and C transduced vessels. Pooled data for the average lumen vessel area of the LV and C groups at day 7 (D7), 14 (D14), and 21 (D21) are shown in B). There is a significant increase in the average lumen vessel area in the LV transduced vessels when compared to C vessels for day 7 (P<0.001) and day 14 (P<0.01). Pooled data for average area of the media and adventitia for LV and C groups at day 7 (D7), 14 (D14), and 21 (D21) are shown in (C). By day 7, there is a significant increase in the average area of the media and adventitia in the LV transduced vessels when compared to C vessels (P<0.05). By day 14, the average area of the media and adventitia is significantly increased in the LV transduced vessels when compared to C vessels (P<0.01). Pooled data for the average cell density in the media and adventitia in the LV and C groups at day 7 (D7), 14 (D14), and 21 (D21) are shown in (D). There is a significant decrease in average cell density in the media and adventitia in the LV transduced vessels when compared to C vessels for day 7 (P<0.01) and day 14 (P<0.001). Pooled data for average intensity of picrosirius red staining of the vessel wall for LV and C groups at day 7 (D7), 14 (D14), and 21 (D21) are shown in (E). There is a significant decrease in average intensity of picrosirius red staining of the vessel wall of LV transduced vessels when compared to C vessels for day 14 (P<0.001). All are 40X. Scale bar is 50-μM. * Indicates the lumen. Each bar shows mean ± SEM of 4-6 animals per group (B-E). Two-way ANOVA followed by Student t-test with post hoc Bonferroni's correction was performed. ^* P<0.05, ^** P<0.01, or ^# P<0.001.