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. 2014 Apr 14;9(4):e94510. doi: 10.1371/journal.pone.0094510

Figure 6. VEGF-A expression is reduced in LV-shRNA-ADAMTS-1 transduced vessels.

Figure 6

A) is the pooled data from the mean gene expression of VEGF-A at the outflow vein after transduction with LV-shRNA-ADAMTS-1 (LV) compared to control shRNA (C) using qPCR analysis at day 7 (D7), 14 (D14), and 21 (D21). This demonstrates that there is significant reduction in the mean VEGF-A expression in the LV transduced vessels when compared to C vessels at day 7 (P<0.01) and day 14 (P<0.001). By day 21, there is a significant increase in VEGF-A gene expression in the LV treated vessels when compared to C vessels (P<0.0.001). B) is representative sections from VEGF-A staining at the venous stenosis of the LV and C transduced vessels at day at day 14. Cells staining brown are positive for VEGF-A. IgG antibody staining serves as negative control. C) shows that there is a significant reduction in the mean VEGF-A staining in the LV transduced vessels when compared to controls by day 14 (P<0.01) and 21 (P<0.001). D) are representative sections from CD-31 staining at the venous stenosis of the LV-shRNA-ADAMTS-1 (LV) and control shRNA (C) transduced vessels at day at day 14. Cells staining brown are positive for CD31. IgG antibody staining was performed to serve as negative control. E) shows that by day 14, there is a significant reduction in the mean CD31 staining in the LV transduced vessels when compared to controls by day 14 (P<0.05). By day 21, there is a significant increase in the mean CD31 staining in the LV transduced vessels when compared to C (P<0.05). All are 40X. Scale bar is 50-μM. * Indicates the lumen. Each bar shows mean ± SEM of 4–6 animals per group (A, C, and E). Two-way ANOVA followed by Student t-test with post hoc Bonferroni's correction was performed. Significant difference from control value was indicated by * P<0.05 or # P<0.001.