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A. Esr1 (ERα) mRNA; B, C. Western analysis of ERα in visceral adipose tissue; D. Immunolocalisation of ERα to adipocyte nuclei (arrowheads) in mesenteric/visceral adipose tissue; E. Concentrations of estradiol in plasma. Statistical analysis calculated using two way ANOVA * = P<0.05, **P≤0.01, ***P≤0.001 (n = 8 in each group).
