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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Neurotoxicology. 2014 Jan 21;41:54–63. doi: 10.1016/j.neuro.2014.01.002

Figure 4. Time course of mitochondrial ROS generation and intracellular GSH levels in SH-SY5Y cells after stretch injury assessed by flow cytometry.

Figure 4

Cells were treated with 25% strain or 50% strain. Mitochondrial ROS and GSH were analyzed by FACS at the time indicated after stretch. In A (25% strain) and C (50% strain), data are expressed as mitochondrial ROS production (MitoSOX Red positive cell population, %). In B (25% strain) and D (50% strain), data are presented as intracellular GSH (mBCl positive cell population, %) normalized against control values. Data in graphs represent mean ± SEM of n = 5 experiments. *P< 0.05 and **P< 0.01 compared with control.