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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Neurotoxicology. 2014 Jan 21;41:54–63. doi: 10.1016/j.neuro.2014.01.002

Figure 6. Effect of stretch on mitochondrial ROS and intracellular GSH levels in SH-SY5Y cells treated with paraquat.

Figure 6

Cells were stretched at 50% strain 2 h before exposure to paraquat. MitoSOX and mBCl fluorescence was evaluated 48 h after paraquat treatment by FACS. A. Representative histograms depict the changes in MitoSOX and mBCl fluorescence in response to the indicated treatment. In B and C, data are expressed as mitochondrial ROS production (MitoSOX Red fluorescence) normalized against control values. Data in graphs represent mean ± SEM of n = 5 experiments. *P< 0.05 and **P< 0.01.