Skip to main content
. 2014 Mar 24;111(14):5331–5336. doi: 10.1073/pnas.1317242111

Fig. 5.

Fig. 5.

miR-199a stably expressed in EOCC suppresses cell migration in vitro. (A) Representative images of scratch/wound assays of A2780 cells expressing GFP or miR-199a/GFP lentivirus are shown. The green lines indicate wound edge (migration). (B) Migration distances were measured from six wells per group, and the average percentage of wound closure was calculated. (C) Real-time migration of A2780 cells expressing GFP or miR-199a/GFP in the presence and absence of hypoxia mimetic DFX. Delta cell index indicates electrical impedance measurements. (D) miR-199a effects on cell migration were mitigated by overexpressing nondegradable form of HIF-1α lacking 3′-UTR. Cells transfected with P-to-A HIF-1α, exposed to normoxia or hypoxia, are shown. (E) Quantification of scratch/wound assay described in D. (F) Real-time migration of A2780 GFP or miR-199a/GFP cells transfected with P-to-A HIF-1α. Values represent mean ± SD. *P < 0.05.