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. 2014 Mar 24;111(14):5331–5336. doi: 10.1073/pnas.1317242111

Fig. 6.

Fig. 6.

miR-199a inhibits peritoneal seeding and tumor burden. (A) Representative images of female athymic mice injected intraperitoneally (i.p.) with A2780 GFP or miR-199a/GFP are shown. (B) The yellow arrows show tumor seeding, and the blue arrows show GI metastases. (C) Representative images of tumor burden. (D) Wet weight of tumors. Values represent mean ± SD. P values are shown in parentheses. (E) LOX transcript levels were determined by qPCR. Each value is a mean of three independent experiments (*P < 0.05). (F) Real-time cell attachment to ECM components was measured by electrical impedance (cell index). C, control; COL I, collagen type 1; FB, fibronectin; LMN, laminin. (G) Effect of LOX inhibitor, β-aminopropionitrile (β-APN) on Transwell migration of cells. DFX, desferrioxamine, 100 μM. (H) Control and miR-199a–expressing A2780 tumor sections were stained for LOX [immunohistochemistry (IHC)] and second harmonic generation (SHG) to evaluate collagen deposition. Representative images are shown.