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Fibrillization kinetics assays and negative-stain EM of HET-s(218–289) WT and double asparagine-ladder mutants. (A) Fibrillization kinetics assay plots: WT (■ and solid line), NAB (▲ and dotted line), and NAD (● and dashed line). Negative-stain EM for (B) WT, (C) NAB at 30 min, (D) NAD at 30 min, (E) NAB at 4 h, and (F) NAD at 4 h. See also Fig. S5. (Scale bars, 100 nm.)
