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. 2014 Mar 21;111(14):5295–5300. doi: 10.1073/pnas.1321427111

Fig. 5.

Fig. 5.

TRAF2 is a key molecule for CD40-mediated IRAK1 sumoylation and nuclear translocation. (A) Co-IP of TRAF2 and IRAK1 after LPS plus CD40 Ab treatment. (B–E) TRAF2 expression (B), IRAK1 sumoylation (C), IRAK1-IRF5 co-IP (D), and IRAK1 nuclear translocation (E) in Traf2 shRNA-transfected macrophages stimulated with LPS plus CD40 Ab for 60 min. (Data in B and C were obtained from the same set of lysates. Therefore, we used the same β-actin blots for the internal control of B and C. Scale bar in E, 5 μm.) (F) IL-10 production in macrophages stimulated with LPS plus CD40Ab for 24 h was detected by ELISA. Final concentrations of LPS and Ab/IgG in tissue culture were 100 ng/mL and 10 µg/mL, respectively. *P < 0.05. Data are presented as mean ± SEM (n = 4 per group).