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. 2014 Mar 24;111(14):5349–5354. doi: 10.1073/pnas.1317731111

Fig. 3.

Fig. 3.

Activation of canonical WNT/β-catenin pathway induces differentiation of human ERMS cells. (A) Graphic summary of MF20-positive cells in human ERMS treated with DMSO or BIO. *Statistically significant differences between experimental and control treated cells (P < 0.05). (B) AXIN2 expression assessed by real-time RT-PCR. (C and D) β-Catenin staining (green) in the presence of DMSO (C) or BIO (D). Blue, DAPI. (Scale bars, 50 μM.) (E) Quantification of MF20-positive cells in human ERMS treated with recombinant WNT3a, CTNNB1 siRNA, and doxycycline-inducible CTNNB1S33Y. *Significant difference for bracketed comparisons (P < 0.05, t test). (F) Western blot analysis.