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Functional characterization of Gal2 wild type and mutants. Growth assay of AFY10X (xylose and xylose–glucose mixtures) and EBY.VW4000 (glucose) overexpressing the indicated transporters. Cells were pregrown in liquid selective SCE or YEPE medium, respectively. Serial dilutions of washed cells were dropped on solid SC media with the indicated carbon sources. Cells were grown at 30 °C for 3 (glucose) or 6 d (xylose and mixtures).
