Fig. 2.

Inhibitory effect of glucose on xylose transport of wild-type and mutant Gal2. Zero-trans influx of xylose was measured in absence of glucose (solid line, filled circle) and in the presence of 25 mM (dashed line, open square) or 100 mM glucose (dotted line, open triangle). EBY.VW4000 overexpressing (A) GAL2 wild type, (B) GAL2-N376F, or (C) GAL2-N376V was grown in selective YEPE. Global curve fitting for Michaelis–Menten kinetics with competitive inhibition was applied to data of three independent measurements at each concentration. Error bars (SEM) may be smaller than the symbols. v, initial rate of uptake.