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. 2014 Mar 24;111(14):5159–5164. doi: 10.1073/pnas.1323464111

Table 2.

Kinetic properties of different Gal2 and Hxt7 mutants and their respective wild type

Transporter Glucose Xylose
Km (mM) Vmax (nmol·min−1·mg−1) Km (mM) Vmax (nmol·min−1·mg−1) Ki (mM)
Gal2
 WT 1.5 ± 0.2 27.2 ± 0.9 225.6 ± 15.8 91.3 ± 3.2 8.6 ± 0.6
 N376F n.d. b.d. 91.4 ± 8.9 37.3 ± 1.3 n.d.
 N376V 22.1 ± 1.8 50.5 ± 1.4 168.3 ± 31.6 28.4 ± 2.3 137.2 ± 45.4
Hxt7
 WT 0.5 ± 0.1 26.0 ± 1.1 200.3 ± 13.2 67.0 ± 2.0 16.9 ± 1.3
 N370S 10.8 ± 1.0 47.3 ± 1.2 169.9 ± 26.3 24.1 ± 1.6 n.d.

Determined by zero-trans influx measurements with transporter-overexpressing EBY.VW4000 and calculated with cell wet weight. SEM is indicated. n.d., not determinable; b.d., below detection limit.