Ten gray of IR induces transient cell cycle arrest, asynchronous apoptosis, and depletion of Olig2+ cells in mouse glioma. Staining for phospho-histone H3 (A), TUNEL (B), Olig2 (C), and Nestin (D). (E) TUNEL+ cells predominantly in tumor bulk rather than Nestin+ perivascular region 24 h after IR. (F) Quantification of Olig2+ nuclei in glioma following IR. Error bars are SD. *P < 0.05, **P < 0.005.