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. 2014 Apr 14;9(4):e94443. doi: 10.1371/journal.pone.0094443

Figure 4. The effects of BBMD3 on non-stem-like GBM tumor cells and normal neurospheres.

Figure 4

(A) Left panel, U87 cells were treated with 0, 1, 3, 5, 8, or 10 µM BBMD3 for 24 and 48 hours, and viability was determined using the MTS assay, as described in the Methods. Right panel, U87 cells were treated with 0, 1, 3, 5, or 10 µM BBMD3 for 48 hours and apoptotic cells were analyzed for Annexin V-FITC reactivity and PI staining by flow cytometry. (B) Left panel shows the morphology of a normal human neurosphere. Right panel shows the viability of normal fetal brain derived and tumor derived neurospheres following 24 hours of treatment with 1, 5, or 10 µM BBMD3. Each experiment was performed in triplicate, and replicated as an independent experiment at least twice. The top of each bar graph represents the mean of the observed values from these replicates, and the error bars denote ± the standard deviation from the mean (SD).