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. 2014 Apr 14;9(4):e94443. doi: 10.1371/journal.pone.0094443

Figure 5. The effect of miR-4284 and miR27a inhibitors on the cytotoxic activity of BBMD3 in PBT003 and PBT030 neurospheres.

Figure 5

(A) Cells from PBT003 and PBT030 neurospheres were transfected with 60 nM of anti-sense inhibitors against miR-4284, miR-27a and an unrelated control miRNA, and the inhibitor was allowed to interact with the miRNA for 48 hours prior to determining the viability of the cells. (B) After cells from PBT003 and PBT030 neurospheres were transfected with 60 nM anti-sense nucleotide inhibitors against miR-4284, miR-27a and an unrelated control anti-sense miRNA, and the inhibitors were allowed to interact with the miRNA for 24 hours; 5 µM of BBMD3 was added to the culture media for another 24 hour. Cell viability was determined at the end of the 48 hour period. Each experiment was performed in triplicate. The top of each bar graph represents the mean of 3 experiments, and the error bars represent ± the standard deviation from the mean SD. *, p<0.01; **, p<0.001.