Table 2.
Characterization of Tissue Responses to Implanted Cell Delivery Vehicles1
| CDV | Group | Inflammation2 | Capsule3 | Vascularization4 | Incorporation5 | Necrosis6 |
|---|---|---|---|---|---|---|
| Si-CDV | HaCaT Injected | 3 | 2.25 | 3 | 3 | 1 |
| Ny-CDV | HaCaT Injected | 2.375 | 2 | 2.75 | 2.25 | 1 |
| No Cells | 2.5 | 2 | 2.625 | 2.25 | 1 |
Hematoxylin and eosin stained histological sections of implants were evaluated using a morphometric scoring system. The scale used for each assessment is described below.
Inflammation was assessed by evaluating sections for the presence of macrophages and foreign body giant cells. Scale is 1 to 4 where 1 = no inflammatory cells and 4 = intense inflammatory response.
Capsule formation was assessed by evaluating sections for the presence of an avascular fibrous capsule. Scale is 1 to 4 where 1 = no capsule present and 4 = thick, dense capsule present.
Vascularization was assessed by evaluating sections for the presence of vascular profiles. Scale is 1 to 4 where 1 = no vascular elements present and 4 = highly vascularized tissue.
Incorporation was assessed by evaluating sections for the presence cellularized tissue in association with and within the porous outer layer of the CDV. Scale is 1 to 4 where 1 = no tissue associated with the device and 4 = complete incorporation of device with tissue.
Necrosis was assessed by evaluating sections for the presence of viable and non-viable tissue. Scale is 1 to 4 where 1 = viable issue and 4 = non-viable tissue characterized by cell debris and/or cells exhibiting programmed cell death/apoptosis..