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. 2014 Apr 14;9(4):e94891. doi: 10.1371/journal.pone.0094891

Figure 6. The regulation of TLR4 signaling is TLR2 dependent.

Figure 6

Caco-2/TC7 cells were pretreated with anti-TLR2 antibodies and then treated with L. amylovorus (L. amy), either alone or simultaneously with ETEC. Control (C) and ETEC (E) infected cells were not pretreated with anti-TLR2 antibodies. The figure shows the densitometric values of Western blots. The relative expression levels of Tollip and IRAK-M were normalized to α-tubulin, whereas the phosphorylated IκBα and p65 were normalized to their corresponding unphosphorylated forms. Values represent means ± SD of three independent assays, carried out in triplicate. *P<0.05 compared with all.