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. 2014 Apr 14;9(4):e94322. doi: 10.1371/journal.pone.0094322

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© 2014 Goodwin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HeLa cells over-expressing B12-HA were visualized by electron microscopy. A. A double-walled membrane body within the nucleus connects to a tube (arrow), which then intermingles with other tubes in various orientations. The cytoplasm is at the lower left. B. Double-membrane body in proximity to the nuclear envelope connects to a complex structure extending into the nucleus. Cytoplasm is at left. C. Simple double-membrane structure extending into the nucleus from an atypical nuclear pore (indicated by the arrow). Cytoplasm is at top. D. Complex double-membrane structure containing two atypical nuclear pores (indicated by the arrows) connected to tubular structures inside the nucleus. Cytoplasm is at top.