Table 2. The efficiency of ESC derivation for tetraploid blastocysts.
Embryos | No. blastocysts | No. ICM outgrowths (%) | No. ESC lines derived (%) |
2n | 38 | 31(81.6) | 30(78.9) |
Type a (4n) | 22 | 21(95.5) | 17(77.3) |
Type b (4n) | 43 | 9(20.9)* | 2(4.7)* |
*χ2-test, P<0.01. All the embryos were produced by crossing B6D2F1 females with Oct4-EGFP males, embryos were recovered at the 2-cell stage (42–46h post hCG injection).