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. 2014 Apr 14;9(4):e94730. doi: 10.1371/journal.pone.0094730

Table 3. The efficiency to obtain ESC mice by tetraploid complementation.

Blastocysts ESCs No. embryos transferred No. surrogates No. live pups (%) No. herniated pups (%)
Type a 2nESCs 415 20 59(14.2) 4(6.8)*
Type b 2nESCs 384 19 41(10.7) 28(68.3)
Type a Co-injection 2nESCs 4nESCs 106 6 12(11.3) 0
Type b Co-injection 2nESCs 4nESCs 52 3 5(9.6) 2(40.0)

*χ2-test, P<0.01.Percentage of live pups (%) = No. live pups/No. embryos* 100%; Percentage of herniated pups(%) = No. herniated pups/No. live pups*100%; Co-injection of 2nESCs and 4nESCs into tetraploid blastocysts, each blastocysts received at least ten 2nESCs and ten 4nESCs.