Figure 3. Effect of CpG-ODN-2006 and miltefosine combination therapy on T-cell proliferation at day 4 and 7 post treatments.

Splenocytes were isolated from all the groups of (A) hamsters (normal, infected and treated) and (B) mice followed by incubation in the presence and absence of concanavalin A (5 µg/mL) or soluble Leishmania antigen (5 µg/mL for both hamster and for mice) at 37°C for 72 h and T-cell proliferation was assessed by incorporation of ³H-thymidine. Three independent experiments were done and each bar represents pooled data (mean ± SD) of five animals and the data represent the means of triplicate wells. Mean ± SD between different groups were calculated by one way ANOVA followed by Tukey's post test using graph pad Prism (version 5.0). Significance: Group II vs normal and all treated groups (*P<0.05, **P<0.01, ***P<0.001 and ns; non-significant).