Table 1. Sequences of forward and reverse primers used for quantitative real time-PCR.
Number | Primer | Primer sequence |
1 | HGPRT forward | 5′ GATAGATCCACTCCCATAACTG 3′ |
HGPRT reverse | 5′ TACCTTCAACAATCAAGACATTC 3′ | |
2 | IFN-γ forward | 5′ GCTTAGATGTCGTGAATGG 3′ |
IFN-γ reverse | 5′ GCTGCTGTTGAAGAAGTTAG 3′ | |
3 | IL-12 forward | 5′ TATGTTGTAGAGGTGGACTG 3′ |
IL-12 reverse | 5′ TTGTGGCAGGTGTATTGG 3′ | |
4 | TNF-α forward | 5′ TTCTCCTTCCTGCTTGTG 3′ |
TNF-α reverse | 5′ CTGAGTGTGAGTGTCTGG 3′ | |
5 | iNOS forward | 5′ CGACGGCACCATCAGAGG 3′ |
iNOS reverse | 5′ AGGATCAGAGGCAGCACATC 3′ | |
6 | IL-10 forward | 5′ TGCCAAACCTTATCAGAAATG 3′ |
IL-10 reverse | 5′ AGTTATCCTTCACCTGTTCC 3′ | |
7 | TGF-β forward | 5′ ACGGAGAAGAACTGCTGTG 3′ |
TGF-β reverse | 5′ GGTTGTGTTGGTTGTAGAGG 3′ |