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. 2014 Apr 14;9(4):e94596. doi: 10.1371/journal.pone.0094596

Table 1. Sequences of forward and reverse primers used for quantitative real time-PCR.

Number Primer Primer sequence
1 HGPRT forward 5′ GATAGATCCACTCCCATAACTG 3′
HGPRT reverse 5′ TACCTTCAACAATCAAGACATTC 3′
2 IFN-γ forward 5′ GCTTAGATGTCGTGAATGG 3′
IFN-γ reverse 5′ GCTGCTGTTGAAGAAGTTAG 3′
3 IL-12 forward 5′ TATGTTGTAGAGGTGGACTG 3′
IL-12 reverse 5′ TTGTGGCAGGTGTATTGG 3′
4 TNF-α forward 5′ TTCTCCTTCCTGCTTGTG 3′
TNF-α reverse 5′ CTGAGTGTGAGTGTCTGG 3′
5 iNOS forward 5′ CGACGGCACCATCAGAGG 3′
iNOS reverse 5′ AGGATCAGAGGCAGCACATC 3′
6 IL-10 forward 5′ TGCCAAACCTTATCAGAAATG 3′
IL-10 reverse 5′ AGTTATCCTTCACCTGTTCC 3′
7 TGF-β forward 5′ ACGGAGAAGAACTGCTGTG 3′
TGF-β reverse 5′ GGTTGTGTTGGTTGTAGAGG 3′