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. 2014 Apr 14;9(4):e94781. doi: 10.1371/journal.pone.0094781

Figure 4. Assessing the phenotype of the APCs and LECs in the lymphatic sinuses.

Figure 4

Within the sinuses in the paracortex and medulla, the PROX1+CD169− LECs expressed the lymphatic marker LYVE1. The majority of PROX1−CD169+ APCs in the paracortical and medullary sinuses lacked LYVE1 expression (A), although rare cells in these sinuses appeared to co-express CD169 and LYVE1 (B). CD169+ APCs in the subcapsular and trabecular sinuses lacked LYVE1 (B). LYVE1 was expressed by a subset of the DCN46+ cells in the medullary sinuses, which are likely to represent the LECs (C). CD169+STAB2− APCs were closely associated with the CD169−STAB2+ LECs in the paracortical and medullary sinuses (D–E). The subcapsular and trabecular sinuses did not express STAB2 (F), although CD169+ APCs were present. Blue represents DAPI staining of cell nuclei (D–F). C,capsule; T, trabecula; S, sinus. Scale bars represent 50 µm (A, C–E) and 100 µm (B, F).