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. 2014 Apr 14;9(4):e94383. doi: 10.1371/journal.pone.0094383

Table 1. List of proteins released by apocrine secretion and detected by antibodies using immunostaining.

Protein Corresponding gene MW (kDa) Function/Cellular localization Detection method Time of release (hr APF)
Actin Act5C + Act42A 41.8 cytoskeletal/cortical, apical antibody/phalloidin 8 and 9.5
Arm armadillo 93.0 cytoskeletal, signaling/membrane, cytoplasmic antibody 9
Baz bazooka 157.4 asymmetric division/cortical, apical antibody 9–10
BR-C Broad-Complex 77.4 transcription and chromatin remodeling factor/nucleus antibody 9
α-Catenin α-Catenin 110.0 cytoskeletal/membrane and cytoplasmic antibody 8
Crb crumbs 234.0 cytoskeletal/apical antibody 9
DHR78 Drosophila hormone receptor in 78 65.4 nuclear receptor, transcription factor/nucleus antibody 9
Dlg discs large 102.0 tumor suppressor/membrane antibody 8–9
Doa Darkener of apricot 55.0 dual-specific protein kinase/cytoplasmic and nuclear antibody 8
E-cadherin shotgun 150.0 cytoskeletal and signaling/membrane antibody 9
EcR Ecdysone receptor 94.0 nuclear receptor, transcription factor/nucleus antibody 8
E63 Ecdysone-induced protein 63F/E63-1 22.0 calcium binding EF hand/cytoplasmic, secretory antibody 8
E74 Ecdysone-induced protein 74EF/E74 87.1 transcription factor/nucleus antibody 9
E75 Ecdysone-induced protein 75B/E75 147.2 nuclear receptor, transcription factor/nucleus antibody 8
Fasciclin I Fasciclin I 72.6 cell adhesion, signaling/cell membrane antibody 9
Fasciclin III Fasciclin III 55.8 cell adhesion, signaling/cell membrane antibody 9
Fibrillarin Fibrillarin 34.6 RNA processing/nucleolus antibody 9
βFTZ-F1 ftz transcription factor1 95.0 nuclear receptor, transcription factor/nucleus antibody 9
Imp-α1 Importin α1 60.0 protein transport/cytoplasmic, nucleus antibody 8
Kr-H Kruppel homolog 1 91.5 transcription factor/nucleus antibody 9
Malic enzyme Malic enzyme 84.0 malate dehydrogenase/cytoplasmic antibody
Met Methoprene-tolerant 79.0 transcription factor/nucleus antibody 9
Mitochondrial pyruvate dehydrogenase l(1)G0334 43.9 pyruvate dehydrogenase/mitochondria antibody 8
Non-muscle myosin II heavy chain zipper 227.0 cytoskeletal antibody 8
Nuclear lamin (T-47) Lamin 76.0 nucleoskeletal/nucleus antibody 10
Numb numb 60.6 signaling/membrane antibody 9
Oho-31 oho31/Pendulin 57.8 transport/nucleus, cytoplasm antibody 8–9
Pan pangolin 81.9 transcription factor/nucleus antibody 9
p53 p53 43.7 transcription factor, tumor suppressor/nucleus antibody 10
p55 Chromatin assembly factor 1 subunit 55.0 chromating remodeling, transcription/nucleus antibody 10
p127 lethal(2)giant larvae 127.0 cytoskeletal and signaling, tumor suppressor/cell membrane antibody 8
Rab11 Rab-protein 11 24.2 GTPase/endosome, trans-Golgi, cytoplasm antibody 10
Ras2 Ras oncogene at 64B 22.2 GTPase/membrane antibody 8
Rop Ras opposite 68.0 transport/cytoplasm, membrane antibody 9
Rpd3 Rpd3 58.3 histone deacetylase/nucleus antibody 9
Rp21 Ribosomal protein 21 M(3)80 26.0 ribosomal protein/cytoplasm antibody 9
Rp40 stubarista 30.2 ribosomal protein/cytoplasm, nucleus antibody 8
Scribbled scribbled 186.0 signaling/cell membrane antibody 9
Sin3A Sin3A 220.0 transcription, corepressor/nucleus antibody 8
Smrter Smrter 379.1 transcription/nucleus antibody 8
α-Spectrin α-spectrin 280.0 cytoskeletal/cell membrane antibody 9
Taiman taiman 215.0 transcription/nucleus antibody 9
Trr trithorax-related 260.0 histone methyltransferase/nucleus antibody 8
α-Tubulin84B α-Tubulin at 84B 49.9 cytoskeletal/cytoplasm antibody 8
β-Tubulin56D β-Tubulin at 56D 50.1 cytoskeletal/cytoplasm antibody 8
Usp ultraspiracle 54.0 nuclear receptor, transcription factor/nucleus antibody 8
Wg wingless 52.0 signaling/membrane, extracellular matrix antibody 9

This table shows 47 proteins identified using laser confocal or fluorescence microscopy of antibody-stained salivary glands. Proteins are listed alphabetically with the corresponding gene name, molecular weight (in kDa), function and predominant cellular localization. The rightmost columns describe the detection method and predominant time of their release into lumen.