Table 1. List of proteins released by apocrine secretion and detected by antibodies using immunostaining.
Protein | Corresponding gene | MW (kDa) | Function/Cellular localization | Detection method | Time of release (hr APF) |
Actin | Act5C + Act42A | 41.8 | cytoskeletal/cortical, apical | antibody/phalloidin | 8 and 9.5 |
Arm | armadillo | 93.0 | cytoskeletal, signaling/membrane, cytoplasmic | antibody | 9 |
Baz | bazooka | 157.4 | asymmetric division/cortical, apical | antibody | 9–10 |
BR-C | Broad-Complex | 77.4 | transcription and chromatin remodeling factor/nucleus | antibody | 9 |
α-Catenin | α-Catenin | 110.0 | cytoskeletal/membrane and cytoplasmic | antibody | 8 |
Crb | crumbs | 234.0 | cytoskeletal/apical | antibody | 9 |
DHR78 | Drosophila hormone receptor in 78 | 65.4 | nuclear receptor, transcription factor/nucleus | antibody | 9 |
Dlg | discs large | 102.0 | tumor suppressor/membrane | antibody | 8–9 |
Doa | Darkener of apricot | 55.0 | dual-specific protein kinase/cytoplasmic and nuclear | antibody | 8 |
E-cadherin | shotgun | 150.0 | cytoskeletal and signaling/membrane | antibody | 9 |
EcR | Ecdysone receptor | 94.0 | nuclear receptor, transcription factor/nucleus | antibody | 8 |
E63 | Ecdysone-induced protein 63F/E63-1 | 22.0 | calcium binding EF hand/cytoplasmic, secretory | antibody | 8 |
E74 | Ecdysone-induced protein 74EF/E74 | 87.1 | transcription factor/nucleus | antibody | 9 |
E75 | Ecdysone-induced protein 75B/E75 | 147.2 | nuclear receptor, transcription factor/nucleus | antibody | 8 |
Fasciclin I | Fasciclin I | 72.6 | cell adhesion, signaling/cell membrane | antibody | 9 |
Fasciclin III | Fasciclin III | 55.8 | cell adhesion, signaling/cell membrane | antibody | 9 |
Fibrillarin | Fibrillarin | 34.6 | RNA processing/nucleolus | antibody | 9 |
βFTZ-F1 | ftz transcription factor1 | 95.0 | nuclear receptor, transcription factor/nucleus | antibody | 9 |
Imp-α1 | Importin α1 | 60.0 | protein transport/cytoplasmic, nucleus | antibody | 8 |
Kr-H | Kruppel homolog 1 | 91.5 | transcription factor/nucleus | antibody | 9 |
Malic enzyme | Malic enzyme | 84.0 | malate dehydrogenase/cytoplasmic | antibody | |
Met | Methoprene-tolerant | 79.0 | transcription factor/nucleus | antibody | 9 |
Mitochondrial pyruvate dehydrogenase | l(1)G0334 | 43.9 | pyruvate dehydrogenase/mitochondria | antibody | 8 |
Non-muscle myosin II heavy chain | zipper | 227.0 | cytoskeletal | antibody | 8 |
Nuclear lamin (T-47) | Lamin | 76.0 | nucleoskeletal/nucleus | antibody | 10 |
Numb | numb | 60.6 | signaling/membrane | antibody | 9 |
Oho-31 | oho31/Pendulin | 57.8 | transport/nucleus, cytoplasm | antibody | 8–9 |
Pan | pangolin | 81.9 | transcription factor/nucleus | antibody | 9 |
p53 | p53 | 43.7 | transcription factor, tumor suppressor/nucleus | antibody | 10 |
p55 | Chromatin assembly factor 1 subunit | 55.0 | chromating remodeling, transcription/nucleus | antibody | 10 |
p127 | lethal(2)giant larvae | 127.0 | cytoskeletal and signaling, tumor suppressor/cell membrane | antibody | 8 |
Rab11 | Rab-protein 11 | 24.2 | GTPase/endosome, trans-Golgi, cytoplasm | antibody | 10 |
Ras2 | Ras oncogene at 64B | 22.2 | GTPase/membrane | antibody | 8 |
Rop | Ras opposite | 68.0 | transport/cytoplasm, membrane | antibody | 9 |
Rpd3 | Rpd3 | 58.3 | histone deacetylase/nucleus | antibody | 9 |
Rp21 | Ribosomal protein 21 M(3)80 | 26.0 | ribosomal protein/cytoplasm | antibody | 9 |
Rp40 | stubarista | 30.2 | ribosomal protein/cytoplasm, nucleus | antibody | 8 |
Scribbled | scribbled | 186.0 | signaling/cell membrane | antibody | 9 |
Sin3A | Sin3A | 220.0 | transcription, corepressor/nucleus | antibody | 8 |
Smrter | Smrter | 379.1 | transcription/nucleus | antibody | 8 |
α-Spectrin | α-spectrin | 280.0 | cytoskeletal/cell membrane | antibody | 9 |
Taiman | taiman | 215.0 | transcription/nucleus | antibody | 9 |
Trr | trithorax-related | 260.0 | histone methyltransferase/nucleus | antibody | 8 |
α-Tubulin84B | α-Tubulin at 84B | 49.9 | cytoskeletal/cytoplasm | antibody | 8 |
β-Tubulin56D | β-Tubulin at 56D | 50.1 | cytoskeletal/cytoplasm | antibody | 8 |
Usp | ultraspiracle | 54.0 | nuclear receptor, transcription factor/nucleus | antibody | 8 |
Wg | wingless | 52.0 | signaling/membrane, extracellular matrix | antibody | 9 |
This table shows 47 proteins identified using laser confocal or fluorescence microscopy of antibody-stained salivary glands. Proteins are listed alphabetically with the corresponding gene name, molecular weight (in kDa), function and predominant cellular localization. The rightmost columns describe the detection method and predominant time of their release into lumen.