Table 3. List of proteins released by apocrine secretion and detected by chromogenic staining.
| Protein | Corresponding gene | MW (kDa) | Function/Cellular localization | Detection method | Time of release (hr APF) | Refe-rence |
| Antp | Antennapedia | 43.0 | transcription factor/nucleus | lacZ | 9 | |
| Arm | armadillo | 93.0 | cytoskeletal, signaling/membrane, cytoplasmic | lacZ | 9 | FBti 0018347 |
| Brk | brinker | 77.5 | transcription factor/nucleus | lacZ | 10 | |
| Capt | capulet | 45.6 | actin binding/cytoplasm | lacZ | 9 | |
| CG14207 (Hsp20-like α-crystallin) | CG14207 | 20.8 | heat shock protein/cytoplasm | lacZ | 9 | FBti 0038459 |
| CG6175 | CG6175 | 61.6 | unknown | lacZ | 8 | |
| CG8668 | CG8668 | 64.7 | glycosyltransferase/Golgi, membrane | lacZ | 8 | |
| Cype | cyclope | 8.3 | cytochrome c oxidase/mitochondrion | lacZ | 9 | FBti 0005248 |
| Dlc90F | Dynein light chain 90F | 12.5 | cytoskeletal/cytoplasm | lacZ | 9 | |
| Doa | Darkener of apricot | 55.0 | dual-specific protein kinase/cytoplasmic and nuclear | lacZ | 8 | FBti 0005439 |
| DX16 (hn RBP1-like GFP-Ping) | x16 | 27.9 | RNA processing/nucleus | lacZ | 8 | |
| Ec | echinus | 188.4 | ubiquitin thiolesterase,cytoplasm | lacZ | 9 | |
| En | engrailed | 59.4 | transcription factor/nucleus | lacZ | 8 | FBti 0002246 |
| Fer2LCH | Ferritin 2 light chain homologue | 25.2 | iron binding/Golgi, secretory | lacZ | 9 | FBti 0005395 |
| Fkh | fork head | 54.0 | transcription factor/nucleus | lacZ | 8 | |
| For | foraging | 101.1 | protein kinase/membrane | lacZ | 9 | FBti 0006974 |
| Fray | frayed | 60.3 | PASK/SPAK kinase/cytoplasmic | lacZ | 8 | FBti 0005585 |
| Int6 | Int6 homologue | 48.0 | translation/cytoplasm | lacZ | 9 | |
| Lab | labial | 67.5 | transcription factor/nucleus | lacZ | 8 | FBti 0005424 |
| LAMP1 | Lamp1 | 34.8 | vesicular/lysosome | lacZ | 9 | |
| Mod | modulo | 60.3 | DNA/RNA binding/nucleus, nucleolus, cytoplasm | lacZ | 10 | FBti 0009927 |
| Ng-1 | new glue 1 | 11.4 | extracellular glue/secreted | lac Z | 8 | * {f} |
| Oda | Ornithine decarboxylase antizyme | 28.3 | enzyme inhibitor/cytoplasm | lacZ | 9 | |
| Pdcd4 | Programmed cell death 4 ortholog | 56.4 | RNA metabolism/cytoplasm | lacZ | 9 | |
| Pnut | peanut | 60.1 | cytoskeletal, GTPase/membrane | lacZ | 9 | |
| Poly(A)-binding protein 2 | Pabp2 | 33.0 | RNA processing/nucleus, cytoplasm | lacZ | 8 | FBti 0071136 |
| Puc | puckered | 58.0 | phosphatase/Golgi, endoplasmic reticulum | lacZ | 8 | |
| Pum | pumilio | 156.0 | translation/cytoplasm | lacZ | 8 | |
| RCC1-like protein | Regulator of chromosome condensation 1 ortholog | 58.9 | chromatin binding/nucleus | lacZ | 10 | |
| RhoGAP71E | Rho GTPase activating protein at 71E | 66.4 | signaling/membrane | lacZ | 9 | |
| RpS27A | Ribosomal protein S27A | 17.9 | ribosomal protein/cytoplasm | lacZ | 9 | FBti 0005278 |
| Sktl | skittles | 87.8 | transferase/cell membrane, membrane | lacZ | 8 | |
| Sply | Sphingosine-1-phosphate lyase | 60.3 | decarboxylase/endoplasmic reticulum, membrane | lacZ | 9 | |
| Sra | sarah | 31.4 | signaling/cytoplasm, mitochondrion, nucleus | lacZ | 10 | |
| Syx13 | Syntaxin 13 | 31.5 | transport/membrane | lacZ | 9 | |
| Tau | tau | 60.0 | cytoskeletal/microtubule | lacZ | 10 | |
| Thor | Thor | 12.9 | translation/cytoplasm | lacZ | 9 | FBti 0009315 |
| Tramtrack | tramtrack | 97.0 | transcription factor/nucleus | lacZ | 8 | FBti 0005154 |
| Tropomyosin 1 | Tropomyosin 1 | 39.3 | cytoskeletal/cytoplasm, cytoskeleton | lacZ | 10 | |
| Twr | twisted bristles roughened eye | 21.0 | peptidase/membrane | lacZ | 9 | |
| vATPase subunit D | Vacuolar H+ ATPase subunit 36–1 | 27.6 | vATPase/vacuole | lacZ | 9–10 | FBti 0006704 |
| VhaSFD | Vacuolar H+-ATPase SFD subunit | 53.7 | vATPase/vacuole | lacZ | 8–9 | |
| Zw3 Ser/Thr kinase | shaggy | 56.0 | protein kinase/cell junction, cytoplasm, nucleus | lacZ | 9 |
Table 3 shows 44 entities detected by positive LacZ staining of P-element insertions, as described under Materials and Methods. Also these proteins are listed alphabetically with the corresponding gene name, molecular weight (in kDa), function and predominant cellular localization. The rightmost columns describe not only the detection method but also predominant time of their release into lumen and whenever possible also genotype reference.
{f} Crispi S, Giordano E, D‘Avino PP, Peluso I and Furia M (2001) Functional analysis of regulatory elements controlling the expression of the ecdysone-regulated Drosophila ng-1 gene. Mech. Dev. 100: 25–35.