Table 1.
Physicochemical Characteristics for Ginsenosides Re (1), Rf (2), Rg2 (3), and 20-gluco-Rf (4)
| Ginsenoside Re | Ginsenoside Rf | Ginsenoside Rg2 | 20-glucoginsenoside Rf | |
|---|---|---|---|---|
| Crystals | Colorless powder | White powder | Colorless powder | White powder |
| Melting point (°C) | 186–187 | 180–181 | 191–192 | 204–205 |
| [α]D | –1.80° (28°C, c = 0.50, MeOH) | +13.80° (28°C, c = 0.50, MeOH) | –3.84° (28°C, c = 0.50, MeOH) | +64.00° (28°C, c = 0.50, MeOH) |
| IR (cm–1) | 3359, 2929, 1642, 1072, 1045 | 3360, 2924, 1637, 1071, 1031 | 3391, 2930, 1635, 1070, 1048 | 3360, 2930, 1635, 1074, 1032 |
| FAB/MS (m/z) | 945, 765, 475, 265 | 799, 475, 325 | 765, 281, 255 | 961, 799 |
| TLC (Rf) | 0.271), 0.572) | 0.371), 0.292) | 0.511), 0.132) | 0.281), 0.652) |
| HPLC (Rt, min) | 27.13) | 20.63) | 10.33) | 30.23) |
FAB, fast atom bombardment; Rf, retention factor; Rt, retention time.
1)
Kieselgel 60 F254, CHCl3:MeOH:H2O (65:35:10).
2)
Kieselgel RP-18 F254S, MeOH:H2O (2:1).
3)
Carbohydrate ES (5 μm, 250 × 4.6 mm), solvent A (acetonitrile:H2O:isopropanol = 80:5:15), solvent B (acetonitrile:H2O:isopropanol = 60:25:15), gradient elution: B 25% at 0–12 min, 25–80% at 12–25 min, 80% at 25–32 min, 80–90% at 32–33 min, and 90% at 33–38 min. Flow rate = 0.8 mL/min.