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. 2014 Apr 3;2(4):491–506. doi: 10.1016/j.stemcr.2014.02.011

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© 2014 The Authors

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

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HTT Codistributes with Dynein/Dynactin/NUMA/LGN

(A and B) Mammary cells stained as indicated. Scale bars, 10 μm. Telo, telophase; Ana, anaphase; Meta, metaphase; Prometa, prometaphase; Pro, prophase; Inter, interphase.

(C) HTT/dynein/dynactin/NUMA/LGN complexes were immunoprecipitated from cells arrested in metaphase before lysis. Mouse IgG (mIgG) was used as a negative control. The immunoprecipitates (IP) were analyzed by western blotting.

(D) Cartoon showing the HTT/dynein/dynactin/NUMA/LGN complex at the spindle pole and on astral microtubules in metaphase cells.

See also Figure S4.