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. 2014 Mar 20;2(4):457–472. doi: 10.1016/j.stemcr.2014.02.001

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© 2014 The Authors

This work is licensed under a Creative Commons Attribution 3.0 Unported License.

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Clonal Analysis of CD11A⁻ and CD11A⁺ KLS

(A) VE-cadherin (VE-CAD; y axis) versus CD11A (x axis) expression of embryonic KLS from e9.5 to e11.5.

(B) Percent of multipotent colonies from clone-sorted CD11A⁻ and CD11A⁺ KLS subsets. The percent of multipotent colonies arising from adult BM KLS is indicated in black and was duplicated from Figure 1E. For e9.5 and e10.5, CD11A⁻ and CD11A⁺ KLS cells derived from the entire embryo proper (EP) were analyzed. The number of multipotent colonies observed out of total colonies scored is listed in parentheses.

(C) Lineage distribution of CD11A⁻ and CD11A⁺ KLS colonies. The distribution of lineages that resulted from clone-sorted CD11A⁻ and CD11A⁺ KLS cells onto ODT stroma from e9.5 to e11.5 is shown. A description of the scoring system can be found in the legend to Figure 1E. The percentage of colonies scored as multipotent is shown as white bars and is identical to the data shown in (B).