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. 2014 Mar 20;2(4):457–472. doi: 10.1016/j.stemcr.2014.02.001

Figure 4.

Figure 4

Numeric Analysis of Hematopoietic Progenitor Cells in Embryonic Development

(A) Absolute number of colony-forming cells per embryo from YS (blue squares), AGM (red downward triangles), and FL (green diamonds) from e8.5 to e11.5. At e8.5 and e9.5, the whole embryo proper (EP; orange upright triangles) was cultured instead of AGM and FL. Unsorted tissues were plated onto ODT stroma at serial dilutions and colonies counted at day 3. The number of litters analyzed (n) for each time point is indicated.

(B–D) The absolute numbers of CD11A− KLS (B), CD11A+ KLS (C), and MYP (D) cells were calculated for YS (blue), AGM (red), and FL (green) from e9.5, e10.5, and e11.5. The numbers shown are per tissue, per embryo. Error bars are SD. The asterisk (∗) in (B) indicates a statistically significant difference (unpaired t test) between the absolute number of CD11A− KLS cells in the YS and AGM at e10.5 (p < 0.002). The number of litters analyzed (n) for each tissue (YS in blue, AGM in red, FL in green) is indicated.

(E) Estimation of the absolute number of clonally multipotent cells per tissue per embryo. These numbers were generated by multiplying the absolute number of CD11A− KLS (from B) times the percent of multipotent cells contained within (from Figure 3B).