FOXO3 Suppresses Cell-Cycle Entry of SCs
(A) SCs from Foxo3−/− and wild-type mice were tested for entry into the cell cycle by the incorporation of EdU at different times. The graph shows the percentage of SCs from Foxo3−/− and wild-type (WT) mice that incorporated EdU in the first 36 hr in culture. A greater number of Foxo3−/− SCs incorporated EdU than wild-type SCs (∗∗∗p < 0.005).
(B) FACS analysis was performed on proliferating SC progeny isolated from Foxo3−/− and wild-type mice. The distribution of SC progeny in G0/G1, S, and G2/M phases of the cell cycle are plotted as a percentage of the total number of cells. The percentage of Foxo3−/− myogenic progenitors in S phase was twice that of wild-type cells (n = 3 mice per genotype).