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. Author manuscript; available in PMC: 2014 Apr 15.

Published in final edited form as: J Periodontol. 2013 Jan 31;84(11):1606–1616. doi: 10.1902/jop.2013.120294

Methylation level of TNFA promoter region and transcriptional level of TNFA in clinical gingival biopsies collected from sites either with chronic periodontitis or periodontal health. A) The percentage methylation of each individual CpG dinucleotide from chronic periodontitis tissues is compared with gingival tissues with periodontal health. “*” indicates significantly higher methylation level at site -163bp in chronic periodontitis samples (p=0.02); “**” indicates significantly higher methylation level at -161bp in chronic periodontitis samples (p=0.04). B) TNFA transcriptional expression from periodontitis biopsies is compared with gingival biopsies with periodontal health. The transcription of TNFA is not significantly different from the samples with periodontal health (p=0.08). C) The messenger level of TNFA of individual sample from both biopsy groups is plotted against its methylation level at site -163bp. Regression analysis indicates that the transcriptional level of TNFA is significantly and inversely related to the methylation level at -163bp (r=0.16, p=0.018).

Methylation level of TNFA promoter region and transcriptional level of TNFA in clinical gingival biopsies collected from sites either with chronic periodontitis or periodontal health. A) The percentage methylation of each individual CpG dinucleotide from chronic periodontitis tissues is compared with gingival tissues with periodontal health. “*” indicates significantly higher methylation level at site -163bp in chronic periodontitis samples (p=0.02); “**” indicates significantly higher methylation level at -161bp in chronic periodontitis samples (p=0.04). B) TNFA transcriptional expression from periodontitis biopsies is compared with gingival biopsies with periodontal health. The transcription of TNFA is not significantly different from the samples with periodontal health (p=0.08). C) The messenger level of TNFA of individual sample from both biopsy groups is plotted against its methylation level at site -163bp. Regression analysis indicates that the transcriptional level of TNFA is significantly and inversely related to the methylation level at -163bp (r=0.16, p=0.018).