Table 1. Oligonucleotides used for bisulfite specific PCR and pyrosequencing.

CpG site (*) Included	Details	Sequences	Annealing T (°C)
-244, -238	Forward	5′-TAGGTTTTGAGGGGTATGGG -3′	62
	Reverse	5′-[Biotin]TCAAAAATACCCCTCACACTCC -3′
	Sequencer	5′-GTTAGTGGTTTAGAAGATTT-3′
-169, -163, -161	Forward	5′-[Biotin]GAGTGTGAGGGGTATTTTTGATG -3′	63
	Reverse	5′-GCAACCATAATAAACCCTACACCTTC-3′
	Sequencer	5′-AAACCCTACACCTTCTATCT -3′
-146, -119	Forward	5′-GAGGGGTATTTTTGATGTTTGTGT -3′	61
	Reverse	5′-[Biotin] CAACCAACCAAAAACTTCCTTAAT-3′
	Sequencer	5′-TTTAGAGATGGAGAAGAAA-3′
-72, -49, -38	Forward	5′-GAGGGGTATTTTTGATGTTTGTGT-3′	63
	Reverse	5′-[Biotin]CCAACAACTACCTTTATATATCCC -3′
	Sequencer	5′-TTATGGGTTTTTTTATTAAG-3′

^*CpG sites indicate nucleotide position in relation to transcription start (TSS).

“−” or “+”, indicates upstream or downstream of TSS, respectively.