Table 3.

Summary of Estimated Diversities Obtained from Pyrosequences

Sample		Analyzed reads	Normalized reads	Observed OTUs	Estimated OTUs (Chao1)	Shannon diversity	Good's coverage
Prior to	1	1,866	1,866	336	1,728.13	3.67	0.86
	2	2,923	2,000	340	3,087.25	3.20	0.85
	3	2,753	2,000	243	1,743.23	2.96	0.90
	4	5,354	2,000	246	1,103.37	3.68	0.91
	5	4,863	2,000	350	1,988.45	3.89	0.87
	6	2,485	2,000	227	595.88	3.63	0.93
	7	3,320	2,000	179	485.71	3.01	0.94
	8	3,334	2,000	213	717.79	3.38	0.93
	9	4,503	2,000	427	3,647.56	4.32	0.83
	10	5,070	2,000	218	784.25	3.37	0.92
After	1	2,056	2,000	372	1,440.03	4.14	0.87
	2	2,262	2,000	343	2,893.88	3.38	0.85
	3	2,953	2,000	244	1,544.20	2.96	0.90
	4	4,922	2,000	297	1,394.29	3.49	0.89
	5	5,137	2,000	522	3,800.79	4.65	0.79
	6	4,345	2,000	415	1,555.36	4.21	0.85
	7	3,209	2,000	229	913.00	3.65	0.92
	8	2,417	2,000	96	255.00	2.59	0.97
	9	3,510	2,000	159	366.83	3.29	0.96
	10	6,329	2,000	163	649.77	2.47	0.94

OTU, operational taxonomic unit.