Table 1.
Peak No2) | Saponin | Molecular Formular | Steaming time |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Raw(0 h) | 2 h | 4 h | 6 h | 8 h | 10 h | 12 h | 14 h | 16 h | 18 h | 20 h | |||
1 | MR1 | C42H72O15 | 35.9 ± 7.0 | 29.9 ± 1.0 | 28.4 ± 1.8 | 25.8 ± 4.4 | 22.1 ± 0.7 | 24.2 ± 0.8 | 24.1 ± 1.0 | 23.6 ± 0.9 | 22.0 ± 1.7 | 20.6 ± 0.6 | 21.3 ± 0.6 |
2 | Rg1+Re3) | C42H72O14 | 30.1 ± 5.4 | 11.6 ± 0.4 | 1.3 ± 0.1 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
3 | MR2 | C41H70O14 | 44.3 ± 6.9 | 39.0 ± 1.1 | 40.2 ± 2.4 | 37.1 ± 5.6 | 30.6 ± 0.4 | 33.1 ± 1.8 | 34.0 ± 1.9 | 31.4 ± 2.7 | 29.6 ± 2.4 | 27.3 ± 2.7 | 25.9 ± 0.8 |
4 | Unknown 14) | 5.4 ± 1.3 | 4.9 ± 0.2 | 6.2 ± 0.8 | 6.3 ± 0.8 | 5.6 ± 0.9 | 7.3 ± 0.1 | 8.0 ± 0.6 | 8.8 ± 0.7 | 9.8 ± 1.1 | 9.9 ± 1.7 | 12.4 ± 1.1 | |
5 | VR1+VR25) | C43H72O15 | 23.5 ± 3.2 | 19.8 ± 0.5 | 18.6 ± 1.6 | 16.3 ± 2.7 | 13.7 ± 0.2 | 14.3 ± 0.7 | 14.5 ± 0.9 | 13.1 ± 0.5 | 12.3 ± 1.0 | 11.0 ± 1.1 | 9.6 ± 0.4 |
6 | Unknown 24) | 11.7 ± 1.9 | 7.2 ± 0.5 | 1.0 ± 0.1 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | |
7 | Rb1 | C54H92O23 | 23.6 ± 3.5 | 14.4 ± 0.5 | 4.5 ± 0.7 | 0.8 ± 0.2 | 0.9 ± 0.4 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
8 | Rc | C53H90O22 | 13.0 ± 2.3 | 7.0 ± 0.3 | 1.5 ± 0.2 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
9 | Rb2 | C53H90O22 | 10.4 ± 1.9 | 5.5 ± 0.5 | 2.1 ± 0.2 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
10 | 20(S)-Rh1 | C36H62O9 | 6.1 ± 1.4 | 13.8 ± 0.8 | 17.5 ± 0.7 | 14.1 ± 2.4 | 12.3 ± 1.2 | 10.8 ± 0.8 | 10.7 ± 0.6 | 7.8 ± 0.8 | 6.2 ± 0.5 | 6.2 ± 1.8 | 3.9 ± 0.4 |
11 | 20(R)-Rh1 | C36H62O9 | N.D.6) | 7.2 ± 0.4 | 11.7 ± 0.8 | 10.7 ± 1.8 | 9.7 ± 0.5 | 10.9 ± 0.7 | 11.0 ± 0.5 | 10.4 ± 0.4 | 9.6 ± 0.9 | 8.8 ± 0.6 | 7.8 ± 0.6 |
12 | Rd | C48H82O18 | 8.2 ± 1.2 | 5.8 ± 0.3 | 3.1 ± 0.3 | 1.4 ± 0.2 | 1.1 ± 0.5 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
13 | Rk3 | C36H60O8 | N.D. | 2.6 ± 1.8 | 7.1 ± 0.7 | 6.8 ± 1.6 | 5.9 ± 0.7 | 7.5 ± 0.6 | 7.8 ± 0.6 | 7.9 ± 0.3 | 7.3 ± 0.5 | 6.9 ± 0.4 | 7.3 ± 0.3 |
14 | Rh4 | C36H60O8 | N.D. | 4.5 ± 0.3 | 8.8 ± 0.7 | 8.7 ± 1.8 | 8.2 ± 0.8 | 10.5 ± 0.6 | 10.4 ± 0.5 | 11.6 ± 0.5 | 11.4 ± 0.8 | 10.7 ± 1.4 | 12.7 ± 0.8 |
15 | 20(S)-Rg3 | C42H72O13 | N.D. | 5.9 ± 0.4 | 13.4 ± 0.7 | 13.8 ± 2.7 | 13.1 ± 0.5 | 15.1 ± 0.7 | 15.3 ± 1.0 | 14.5 ± 0.7 | 13.5 ± 1.0 | 12.8 ± 0.4 | 11.9 ± 0.3 |
16 | 20(R)-Rg3 | C42H72O13 | N.D. | 4.6 ± 0.3 | 11.3 ± 0.8 | 11.7 ± 2.4 | 10.8 ± 0.5 | 13.0 ± 0.9 | 13.2 ± 0.7 | 13.0 ± 0.9 | 12.9 ± 1.0 | 12.2 ± 0.8 | 13.2 ± 0.6 |
17 | Rk1 | C42H70O12 | N.D. | 2.3 ± 0.2 | 6.1 ± 0.4 | 6.4 ± 1.4 | 5.8 ± 0.6 | 7.2 ± 0.4 | 7.3 ± 0.4 | 7.5 ± 0.4 | 7.1 ± 0.6 | 6.8 ± 0.5 | 7.7 ± 0.6 |
18 | Rg5 | C42H70O12 | N.D. | 3.0 ± 0.2 | 7.4 ± 0.5 | 7.8 ± 1.9 | 7.1 ± 0.6 | 9.2 ± 0.5 | 9.0 ± 0.5 | 9.4 ± 0.7 | 9.1 ± 0.6 | 8.8 ± 0.8 | 10.5 ± 0.7 |
Sum | 212.4 | 188.8 | 190.1 | 167.8 | 146.7 | 163.2 | 165.3 | 159.1 | 150.8 | 142.0 | 144.2 |
Results are expressed as mean ± SD (n = 3) as mg/(g dried VG).
Peak No. in Fig. 3.
Calculated as Rg1.
Concentration of unknown 1 and 2 were calculated by comparing ELSD responses to MR2 and Rb1, respectively.
Calculated as VR2.
N.D: not detected.