Fig. 4.
Bongkrekic acid inhibits MPT induced by downregulation of endogenous CKMT1. HeLa cells were transfected with siRNA against CKMT1 (siCK1) or a scrambled sequence (sc), and were treated with the indicated amount of bongkrekic acid (BA) after 24 h. (A) MPT was assessed by using DiOC6 and PI co-staining, and FACS analysis at 72 h post transfection. (B) Apoptosis was assessed by annexin-V staining and subsequent FACS analysis. nt, non-treated. The data show the mean±s.d. *P<0.05; **P<0.01; ***P<0.001 (Student's t-test).