Table 1. Validation of the support vector machine (SVM) model using testing data. The SVM model enables the assessment of cell viability and cell death modalities in Saos-2 and SW-1353 cells based on Raman spectroscopy. The confusion matrix depicts the number of spectra that are assigned to each group. Sensitivity, specificity and the total accuracy for the Raman spectral approach are calculated. Annexin V and PI fluorescence staining served as a control method.

		Cell state determined by fluorescence staining
		viable	early apoptosis	late apoptosis	necrosis
	Sum of spectra	89	91	68	85
SVM prediction	viable	89	0	0	0
	early apoptosis	0	87	15	1
	late apoptosis	0	3	51	4
	necrosis	0	1	2	80
	sensitivity [%]	100	96.7	75.0	94.1
	specificity [%]	100	93.4	94.1	97.2
	total accuracy [%]	100	94.3	92.8	97.9