Figure 5. Reduction of FASN mRNA levels by Peretinoin in a human hepatocyte cell line and the effects of ATRA, 9-cis RA, and 13-cis RA on the expression of FASN mRNA.

(A) An immortalised human hepatocyte cell line, THLE-5b cells, was treated with the indicated concentrations of Peretinoin. At 72 h later, RNA was extracted and reverse transcribed, and the levels of FASN mRNA and 18S rRNA were quantified by RTD-PCR. The relative amount of FASN mRNA normalised to that of 18S rRNA is presented as fold change compared to DMSO-treated cells from 3 independent experiments at the indicated conditions. (B) Huh-7.5 cells were transfected with H77S.3/GLuc2A RNA. At 72 h later, the transfected cells were treated with DMSO or 10–40 μM ATRA, 9-cis RA, and 13-cis RA. At 72 h later, RNA was extracted and the levels of FASN mRNA and 18S rRNA were quantified by RTD-PCR. The relative amount of FASN mRNA was determined as described in Fig. 4 and presented as fold change compared to DMSO-treated cells from 3 independent experiments at the indicated conditions.